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Posted by on Jun 25, 2013 in Science |

Reveal surprising technique to clarify biological tissues

Reveal surprising technique to clarify biological tissues

(C) Nature (2013)

A team of neuroscientists led by Meng Ke-Tsen RIKEN Center in Kobe, Japan, developed a new technique ‘very simple and efficient’ to reduce the scattering of light as it passes through biological tissue, which deliver great facilities for the study of the brain and other organs.

See technique called Deep Brain (SeeDB) and consists of a liquid solution that combines fructose, water and small amounts of other compounds. By dipping (or “marinate”) for about three days opaque biological tissue, it is clarified in a rather surprising.

While this is not the only method to clarify biological tissues, the major advantage, according Ke-is that SeeDB is compatible with lipophilic dyes commonly used to map neurons formaldehyde preserved tissue, something that no other methods.

Reveal surprising technique to clarify biological tissues image 2

Links:

SeeDB: a simple and morphology-preserving optical clearing agent for neural circuit reconstruction (Nature Neuroscience)

A Simple Sugar Solution Can Make Tissue Transparent (Gizmodo)

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